I am using the DIG RNA labeling kit to generate an RNA probe for in situ hybridization. I synthesized the probe template via 2-step RT-PCR and purified the template using EXOSAP-IT. According to Nanodrop readings the template had good yield and decent purity (A260/280 was 1.7). After labeling 1ug, I ran an aliquot on a bleach gel and precipitated the remainder. The bands on the gel were quite weak and there was a double band despite treatment with DNase I. There was a very small pellet after precipitation. After hydrolysis and precipitation I could no longer see a pellet and nothing showed up on a gel. Any suggestions?