Kimberley Hobbs

2 Questions 3 Answers 0 Followers

Questions related from Kimberley Hobbs

Has anyone used EXOSAP-IT to clean up PCR products for use as a template for RNA probe synthesis via in vitro transcription?

I am generating RNA probes for in situ hybridization in mussel mantle tissue and am having poor DIG-labeling efficiency. I extracted the RNA using the RNeasy Mini Kit and had good quality RNA. I...

05 May 2021 8,508 5 View

Anyone having trouble with yield from DIG RNA Labeling Kit?

I am using the DIG RNA labeling kit to generate an RNA probe for in situ hybridization. I synthesized the probe template via 2-step RT-PCR and purified the template using EXOSAP-IT. According to...

16 April 2021 2,732 0 View