Theoretical computer virology ? is still alive ?

HI Everyone! I am asking to know by you, expert in cybersecurity and mathematics, if Computer virology (the one of Cohen and Andler) is still an active field of research. That research made in...

08 February 2021 7,613 6 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

How do I organize items to repeated surveys for quantitative and qualitative analysis?

Hello fellow researchers! Newbie here :) I created a survey with open-ended and Likert-type questions. The survey was administered nine times to the same people using google forms. I plan to...

01 January 2021 3,921 3 View

Is there a better library or function for plotting CFA with many observed variables in R?

Hello! I'm kind of new in using R for doing EFA, CFA, and SEM. I just followed a guide to perform CFA on my data to see how my context fits the ITC-SOPI model in a VR photo-based tour. I used...

23 December 2020 1,679 2 View

Best way to validate CRISPR-Cas9 editing efficiency?

I'm trying to obtain genetic KO in my cell line of interest using Lentiviral-mediated CRISPR-Cas9 delivery system. I would like to test more than one unpubblished sgRNA targetting a couple of...

10 August 2020 1,324 4 View

How to convert .txt into plink files (ped/map or bed/bim/fam)?

I have a huge oncoarray_genotype_XXXX.txt file with 11.7 GB size. I would like to convert this file into adequate files for analyzing in plink software. However, I could not find how to conduct...

08 July 2020 9,302 2 View

Fluorescence labeled antibody?

Hi all, I have a small question for you. Is there any fluorescent labeled antibody available which emits the fluorescence only if bound to substrate? So the unlabeled version does not emit any...

19 March 2020 5,715 2 View

How to evaporate Methyl Acetate, and purge added Ethanol?

To use an inhibitor, prepared and sold in a methyl acetate solution, I need to evaporate the aforementioned solvent and exchanging to ethanol or DMSO. I will do that with a gentle nitrogen stream....

01 March 2020 6,947 3 View

SPSS, data with 5 replicates. How to generate the variables-data sheet?

I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...

08 January 2020 3,526 3 View

How to solve multicollinearity problem in mixture design?

Due to its own nature mixture design models (mainly with interactions) presents multicollinearity problems, some authors declare that a combination of forward stepwise and step backward stepwise...

05 January 2020 1,136 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View