I have a problem with some sequencing results (Sanger seq., germinal DNA changes) in one exon.
I´ve obtained different results (SNP - normal allele/ heterozygote) depending on the sense of the primer. I repeated it 3-times (including new amplification) and it´s still the same. This potential change is known as nonpatogenic polymorphism, but not in Caucasians as far as I know.
Thanks.
Larissa Benavente
You might be having allelic drop out. Did you check for possible SNPs in the region where your primers anneal?
1 votes
0 thanks
Gloria Ribas
it may be due to the different chemistry in which the heterozygote position is in the different sequencing direction. Guanines (G) usually have small peaks in the sequence. I don't know where do you have your change it may be explain that way!
1 votes
0 thanks
- Badges
- Science method
More Marketa Hejnalova's questions See All
Similar questions and discussions