How to determine implicit ontological and epistemological stance?

I am hoping for some guidance and additional reading material that will assist me in determining ontological and epistemological stance when not explicitly detailed within the research. Here is...

26 February 2021 227 5 View

How to interpret cyclic voltammogram of an organic material to find HOMO and LUMO values?

Cyclic voltammogram of material with 3 electrode system (Ag/AgCl reference, Pt working electrode and Pt wire as the counter electrode) in 0.1 M Tetra -n butylammonium tetrafluoroborate in...

04 February 2020 214 3 View

What thresholds should I use for common variance/how do I interpret scores (i.e. good, fair, poor)?

I am trying to describe if common variance is good, fair or poor and need a reference or idea of what is acceptable. for example Comrey and Lee (1992) have suggested guidelines/cutoffs: .71...

29 May 2018 5,186 3 View

Does DAPI require permeabilisation?

Hi all,I just attempted a DAPI staining and got absolutely no results from the microscope. The obviously answer would be to increase the DAPI concentration, however I was wondering whether DAPI...

24 September 2017 6,738 9 View

Please does anyone have any idea of what exactly i can do with my control data that would allow my plotted data to start from a single point?

I am doing a research on the effect of 4 polyphenols on kainic acid treated glioblastoma cells. I used a multiplate reader machine to measure cellular proliferation of the 4 polyphenols at...

05 August 2016 6,540 1 View

How can i find the color temperature of an image from its RGB values?

I need to find the exact color temperature value from image's RGB matrix value. I am able to find the x,y as well as u,v (chromaticity space) average matrix values. i am working in labview

21 February 2016 8,645 4 View

In the absent of a rainfall simulator, how can you measure soil erodibility in situ?

Field experiment.

16 September 2014 3,886 12 View

Out of field in relation to integrated learning

Looks like a really timely project. Will you gather - or do you have - data on the contribution made to out of field teaching stats by those teachers who are working in team contexts on integrated...

01 January 1970 5,223 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View