Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

29 January 2021 1,878 4 View

How to correct a deviation in the molar ratio in an Isothermal Titration Calorimetry equipment?

Our equipment of ITC does not reach the molar ratio (N=1) of a titration of EDTA with Ca2+ in MES buffer pH 6. We have cleaned with DCN 90 and HCl pH 2, but the N value is very low, around...

10 November 2020 3,137 3 View

How can I obtain the cohesive energy density and solubility parameter for a crystal using the Materials Studio software?

Hello, I am currently analyzing properties of some minerals such as Bi2O3 by MD using Materials Studio, I need to obtain the cohesive energy density (CED) to obtain the solubility parameter,...

02 November 2020 4,356 1 View

In which solvent should I reconstitute mitoTEMPO?

Dear colleagues, I was wondering which solvent should I use to reconstitute mitoTEMPO, I've seen I could use water or DMSO but I'm not sure about the stability of the compounds in the respective...

18 May 2020 2,882 3 View

Does anyone knows if is there any open source repository of historical aerial photography that includes the north of Chile?

My PhD includes a remote sensing survey of the Atacama Desert using freely available satellite imagery to detect archaeological sites. So far, I have not found any source of historical aerial...

12 May 2020 1,383 8 View

Converting Large Chemical Formats to .pdbqt for AutoDock Vina?

I am trying to convert a large single file library (.sdf, .pdb) containing 50k+ molecules into a .pdbqt file for screening. I have tried using AutoDock Tools but it crashes upon trying to load the...

26 April 2020 5,020 6 View

Do you think the approach to sustainability and environmental issues will change for human society after this pandemic?

We need to be closer to the nature and don't look for money and other values.

29 March 2020 1,238 4 View

Which statistical analysis should I follow for analyzing the effects of two factors in two different cell types cultured in vitro?

Hello all! I have performed experiments involving two different cell types. These two cell types have been cultured in vitro and measurements have been performed after 4, 7 and 10 days of culture....

18 March 2020 6,337 10 View

Can Bacteria grow in DMSO 99%?

I used to test antiproliferative activities of chemical compounds dissolved in DMSO. I have been surprised when testing one of this compounds in HBL-100 cell line bacteria contamination appears. I...

13 February 2020 4,542 2 View

Does anyone know how many samples can I sequence with the minION basic starter pack ?

02 December 2019 3,228 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View