Visualsonics fujifilm exploration in Neurscience for studying striatum, substantia nigra of mice brain on VEVO 3100?

Dear Experts, I am new to animal imaging having VEVO 3100 model of visualsonics, Fujifilm. I wanna ask the procedure of doing it on model VEVO 3100. My region of choice is striatum, substantia...

07 August 2018 7,545 0 View

How much thickness of coronal section of mice brain should be cryosection it using cryostat?

09 October 2016 8,704 7 View

On running protein samples on SDS PAGE I found bromophenol blue color turned to yellow with bad smile???

I runned twice the western SDS Page with my protein samples in 4x lamelli buffer (diluted to 1x with 1X TBS in protein sample lysate) but despite getting blue front of bromophenol blue. I found...

04 May 2016 6,398 6 View

What can be assessed by administering a test drug before the induction of disease?

Usually, for animal model development we first induce disease in animal and then, we give the test compound to cure the induced disease and observe the effect. But in some texts I have read they...

03 April 2012 8,772 1 View

Cell Based Assays........

Can some one tell me about Cel base assays ?? How it is different from invitro assays???? Plz tell me in the brief for understanding....... Thanks

01 February 2012 8,121 5 View

I am new in pharmacology. Can anyone tell me Animal models for Hypertension, Arythmias, Neurobehavioural sciences??

Animal models...

31 December 2011 6,152 24 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View