I m working on coronal section of swiss albino mice strain brain for studying some aspects of immunofluorescence on hippocampal portion, could anybody help me with

 How much thickness of coronal section of mice brain should be cryosection it using cryostat?

or how much thickness of coronal section, I should cut with cryostat so that it can be placed proper on APTS coated slides and process further for imaging.??

Yesterday, after washing in pbs to remove freezing medium. All sections taken on slides got removed.

Please help me if somebody have experience and share there best protocol cum experience to get out this problem.


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