yes, you can reprobe it with primary antibody . but before it, you can wash membrane with wash buffer for 3-4 minutes. and then reprobe with primary antibody( overnight). 

best of luck

Hina

Yes, you can. But it won't intensify your band, if in fact there is no. It could increase the background, so wash properly.

I agree with Wassim's response totally. Washing couple of times with the wash buffer would reduce the background from the first exposure to primary and secondary antibody and help exposure of the same blot for the second time. Good luck.

I do that quiet often. I deshybridize the membrane and re-blot it with the same or different antibodies. The only limitation is that you can not quantify after doing this because the signal is additive from one labeling to another.

Thanks everyone, I decided not to reprobe because it turns out that the transfer was not very successful hence, the poor bands (I stained the gels with Coomassie and found low molecular weight bands on the gels).

Yes, you can do. I would recommend to make a fresh dilution of your antibodies. Wash your membrane a few times, then go on with the primary antibody as usual. In case your primary antibody was bad the first time, you will most probably get bands. But I also agree with Patricia: If you want to quantify be very carefull with the results! Especially in case that your secondary antibody is fine itself but the label is not functional anymore, the antibody will block binding sites without giving a signal.