Elena Zaaba

5 Questions 12 Answers 0 Followers

Questions related from Elena Zaaba

Anyone ever tried reprobing a (Western) blot using the same primary before?

Hi, I ran two gels yesterday (Beta Actin) and did not get good bands. I am very sure that it has nothing to do with my pipetting (I have ran the same samples before and got good bands), therefore...

02 February 2016 4,924 6 View

Why is there no high molecular weight proteins on my (Western Blot) gel?

Hi, I have ran a few gels in order to get a nice Beta actin bands on the blot but have repeatedly failed (did not get a result/nice bands). The samples that I am running are rat lenses. In order...

02 February 2016 6,319 14 View

Low molecular weight proteins are not getting transferred! Help!!

Hi, I ran two gels couple of days ago and did not get good bands. I initially thought that it was my secondary that's not working well. But when I checked the gels I realized that there were thick...

02 February 2016 9,453 7 View

Can anyone help with the problem dissolving protein pellet in 1% SDS + 8M Urea?

Hi, I have been trying to dissolve retina and lens' protein pellets unsuccessfully for the past couple of weeks. Protein was extracted using trisol and everything went well until the dissolving...

10 October 2015 1,234 13 View

How long can a sample be kept in RNA later before RNA extraction?

Does keeping a sample in RNA later too long affect the yield and purity of the RNA extracted?

09 September 2015 2,479 3 View