Does keeping a sample in RNA later too long affect the yield and purity of the RNA extracted?
what sample??? blood or tissue or culture... ???
my suggestion... for RNA extraction u can not store samples long time.. u isolate RNA.. immediately convert into cDNA... because u can store cDNA long time...
Hi,
The yield and purity should not be affected as long as you follow the manual recommendations.
Thank you for the replies. The samples were rat's lenses and retinas. I was asked to do RNA extractions on these samples and the results were not good (low yield and low purity).
Seeing that I am new in RNA extraction, my first impulse was to troubleshoot my technique (rather than to blame on the samples). After spending 2 days trouble shooting (did RNA extraction on other samples that are known to produce good yield and purity & did another extraction on given sample and known sample). The results I got showed that low yield and low purity had nothing to do with my technique.
Therefore, I wanted to figure out what actually caused low yield and purity. I was also told that the lenses and retinas were harvested last June.
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