I am culturing OVCAR-4 cells in RMPI 10% FBS and 1% pen/strep and supl with glut and HEPES the cell viability is low.
It doesn’t seem that you have problem with the media because I use the same as your culture media and supplements and get pretty good viability. It could be something related to cells.
Regard
Thanks
As an answer to my own question, my OVCAR-4 cells grow much much much better in ham's F12-DMEM media (75% F12 and the rest DMEM).
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