Dear Noorjahan A,

can you say more precisely what kind of pigments would you like to extract? Extraction procedure may vary depending on the nature of fungal pigment e.g. to some black fungi containing melanin alkaline extraction is recommended while some other pigments may be extracted using water from other fungal tissues. Some fungi excrete  extracellular pigments to medium.

regards

Hello , Noorjahan A.

You must determine or delimit the pigment ,s kind to know the extraction ,s procedure , because there are many variation among fungi to produce pigments in the media.

With my best wishes...

Dear Noorjahan,

Regarding pigmentation in fungi, you should read some review of literature. a simple Summary of information you may find on following

http://www.fungi4schools.org/Reprints/Mycologist_articles/Isaac_answers/V08pp178-179colour.pdf

There are many methods of extraction based on nature of pigments, but the standard process remains unchanged except the solvent:

After an incubation period of 6 wk, the mycelium is harvested, and filter the supernatant in a sterilized muslin cloth.

Two volumes of 95% (v/v) ethanol is added to the culture broth according to the following procedure:

(i) Dilute with about 60% of the solvent volume, the resulting mixture is kept on the rotary shaker at 180 rpm at 30 C for 30 min;

(ii) Centrifuge the ethanolic mixture at 3780 rpm for 15 min;

(iii) Once the supernatant is recovered, the residue is dispersed in the remaining volume of ethanol and centrifuge again at 3780 rpm for 5 min; and

(iv) the supernatants is then collected and filtered through a pre-weighed Whatman filter paper (47 mm) and further dilute with 95% (v/v) ethanol to a final volumetric dilution factor of 20.

Next, Observe the absorption spectrum at 300–600 nm using UV spectrometer. The purified pigments are concentrated in a rotary evaporator and lyophilized to obtain powder.

you can also read some papers on pigment extraction in fungi

http://www.ugcfrp.ac.in/images/userfiles/63496-Natural.pdf

Hello Noorjahan

Some references 1

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwietaKmpOnYAhUBfywKHXJeA7gQFgguMAA&url=http%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS0144861709004202&usg=AOvVaw08BpnQI-veJZe5CjYo3JQ4

2

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwietaKmpOnYAhUBfywKHXJeA7gQFgg9MAI&url=http%3A%2F%2Fjournals.sagepub.com%2Fdoi%2Fpdf%2F10.1177%2F0040517508087680&usg=AOvVaw1UDizJzAsiIggiSBU7WiCk

3

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=0ahUKEwietaKmpOnYAhUBfywKHXJeA7gQFghCMAM&url=https%3A%2F%2Fpdfs.semanticscholar.org%2F78c8%2F4bf9474e40cdc6aa27ba0e3b7331a760e8fd.pdf&usg=AOvVaw1hI97kUaaeerkSMhdQy0MU

4

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=0ahUKEwietaKmpOnYAhUBfywKHXJeA7gQFghKMAQ&url=http%3A%2F%2Fciteseerx.ist.psu.edu%2Fviewdoc%2Fdownload%3Fdoi%3D10.1.1.862.7874%26rep%3Drep1%26type%3Dpdf&usg=AOvVaw3tvFZYfrFKnufcxMw_un-n

5

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&cad=rja&uact=8&ved=0ahUKEwietaKmpOnYAhUBfywKHXJeA7gQFghnMA0&url=https%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2FBF02931348&usg=AOvVaw0wAdZnMwyRQQ1a6ujzfd8o

6

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=15&cad=rja&uact=8&ved=0ahUKEwietaKmpOnYAhUBfywKHXJeA7gQFghvMA4&url=http%3A%2F%2Fpubs.acs.org%2Fdoi%2Fpdf%2F10.1021%2Fja01578a038&usg=AOvVaw2ur2WaSVROQYu_4niTZWnn

7

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=16&cad=rja&uact=8&ved=0ahUKEwietaKmpOnYAhUBfywKHXJeA7gQFgh3MA8&url=http%3A%2F%2Fwww.tandfonline.com%2Fdoi%2Ffull%2F10.1080%2F21501203.2017.1302013&usg=AOvVaw32BSzVBpCotr8bQuLmST04

8

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=17&cad=rja&uact=8&ved=0ahUKEwietaKmpOnYAhUBfywKHXJeA7gQFgiAATAQ&url=http%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2F10.1111%2Fcote.12080%2Fabstract&usg=AOvVaw2uJkjY7B9yDg_7UXoD2Gml

9

http://www.mdpi.com/2309-608X/3/3/34/pdf

10

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=12&cad=rja&uact=8&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChAWCC4wAQ&url=https%3A%2F%2Fwww.degruyter.com%2Fdownloadpdf%2Fj%2Fbotm.1995.38.issue-1-6%2Fbotm.1995.38.1-6.413%2Fbotm.1995.38.1-6.413.xml&usg=AOvVaw01jbXlCxZCciAxnJnLCqkr

11

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChAWCDYwAg&url=https%3A%2F%2Fwww.slideshare.net%2FArkaBrataBandyapadhyay%2Fmagenta-pigment-isolation-from-fungus&usg=AOvVaw3F8cE19FkikA-ijrMaMB7U

12

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&cad=rja&uact=8&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChAWCD0wAw&url=http%3A%2F%2Fmic.microbiologyresearch.org%2Fcontent%2Fjournal%2Fmicro%2F10.1099%2Fmic.0.000168%3Fcrawler%3Dtrue%26mimetype%3Dapplication%2Fpdf&usg=AOvVaw3SZz2odoPJth-a-RRA-REA

13

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=15&cad=rja&uact=8&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChAWCEYwBA&url=http%3A%2F%2Faem.asm.org%2Fcontent%2F78%2F24%2F8623.full&usg=AOvVaw0z8yhfVSRJmmiHq8_WpO3k

14

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=16&cad=rja&uact=8&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChAWCE4wBQ&url=https%3A%2F%2Fwww.hindawi.com%2Fjournals%2Fjcoat%2F2014%2F759073%2F&usg=AOvVaw1E52YFA1-NZgclhkj72_TW

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=17&cad=rja&uact=8&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChAWCFQwBg&url=https%3A%2F%2Fir.library.oregonstate.edu%2Fdownloads%2Fhh63t1679&usg=AOvVaw3giGnpLic1S4KuVY4ZOa9e

16

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=18&cad=rja&uact=8&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChAWCF0wBw&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC98693%2F&usg=AOvVaw3Qs13YE_1w5vz3uAwDMjQh

17

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=19&cad=rja&uact=8&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChAWCGYwCA&url=https%3A%2F%2Fwww.intechopen.com%2Fbooks%2Fmelanin%2Fproduction-of-melanin-pigment-by-fungi-and-its-biotechnological-applications&usg=AOvVaw2cIykhdLmcCrn394nJvEXL

18

https://books.google.iq/books?id=vP1B3SRE824C&pg=PA293&lpg=PA293&dq=pigmentation+fungi,+extraction+procedure&source=bl&ots=dHFjjVkzgX&sig=GmKPpFeDP8pE7GIzf1YzpOYROGE&hl=en&sa=X&ved=0ahUKEwjH-5WVpenYAhXK1SwKHf5bDuQ4ChDoAQhvMAk

19

https://books.google.iq/books?id=36FmDAAAQBAJ&pg=PA325&lpg=PA325&dq=pigmentation+fungi,+extraction+procedure&source=bl&ots=nCwlpQBh1l&sig=TQe4_8ScafC-5FBVb8P6FZEE-fI&hl=en&sa=X&ved=0ahUKEwjp3IL4punYAhUxhqYKHdO5Cvg4FBDoAQgmMAA

20

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=22&cad=rja&uact=8&ved=0ahUKEwjp3IL4punYAhUxhqYKHdO5Cvg4FBAWCCgwAQ&url=https%3A%2F%2Ffungalbiolbiotech.biomedcentral.com%2Farticles%2F10.1186%2Fs40694-017-0033-2&usg=AOvVaw0yjPuHIDoLADe3t6xV19Kh