Anyone can provide help with shRNA knockdown in H1 (or any other) hESC?

Heshani Nayanga Thirimanne @Heshani-Thirimanne-3

20 June 2018 1 642 Report

I haven't used hESC before. I have a lentivirus construct that I've used in differentiated cells to knockdown the same protein.

What I should do differently in hESC compared to differentiated cells? Media, duration of treatment etc.? Should I look for background differentiation of stem cells?

Joseph Papamatheakis

Ι don't se any reason why a shRNA not to be working in ESC. By the way is the construct working in other cell types? What is the promoter driving expression

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