Heshani Nayanga Thirimanne

2 Questions 2 Answers 0 Followers

Questions related from Heshani Nayanga Thirimanne

Anyone can provide help with shRNA knockdown in H1 (or any other) hESC?

I haven't used hESC before. I have a lentivirus construct that I've used in differentiated cells to knockdown the same protein. What I should do differently in hESC compared to differentiated...

20 June 2018 643 1 View

A biochemical cell fractionation protocol (cytoplasmic, soluble nuclear fraction and chromatin bound fractions)?

I want to determine the cellular localization of a protein, hence looking for a protocol to fractionate cell extracts, i.e. cytoplasmic, soluble nuclear extract and chromatin bound fraction. I...

26 May 2017 7,026 3 View