Hi Chet,

Have a look for links below may help you!

http://link.springer.com/article/10.1007/s13592-015-0377-7

http://link.springer.com/article/10.1007/s13592-012-0122-4

Good luck

Ali

 Thank you Ali.

Regards,

Chet Bhatta, PhD Candidate, 

University of Kansas, lawrence, Kansas

We are using Lep F and Lep R primers for Co I gene, the PCR did not work out well. It gives band only for 10 % of samples although DNA concentration looks fantastically good. Any info on this will be really appreciated. Thanks.