FGT poverty index derivation?

Can anyone show me how the squared poverty gap index can be rewritten as H/n*[u2+(1-u2)CV2)] as in the picture. Step by step please. I have been struggling trying to figure out how to decompose...

07 September 2020 6,867 8 View

Can we run an EFA before a SEM?

I'm conducting a study for my PhD thesis. I've got some constructs from the literature, but the items to measure the constructs were modified (some were added and some were removed). In this case,...

08 June 2020 5,917 9 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

11 September 2019 9,441 2 View

How does freezing antibiotics result in loss of activity?

I remember my mentor saying before that she decided to refrigerate her gentamycin stock solution instead of freezing it since that was the recommended storage condition. Now, I'm dealing with...

04 August 2019 5,766 4 View

Using 5-FC for plasmid curing - confirmation steps?

To cure my Streptomyces strain of a plasmid, I used 5-FC as a negative selection marker. So, cells without the plasmid (which are the ones I'm looking for) will be able to grow on agar plates...

17 July 2019 5,854 2 View

CRISPR base-editing in bacteria not working?

I am currently using the CRISPR base editing system in bacteria. Unfortunately, I'm not getting any clones with edited bases (not even off-target) but i'm not sure why. I'm quite sure the plasmid...

29 May 2019 8,845 5 View

GDNA stored at -20?

I've extracted my gDNA from Streptomyces today, on a Friday but it's pretty late and I can't stay late today. Is it okay for me to keep my extracted gDNA over the weekends at -20 degrees Celsius...

11 April 2019 3,139 9 View

Volume of ligated product for transformation?

I've just done ligation for my plasmid and my next step would be to perform heatshock transformation on Top10 E. coli cells. Some of my colleagues recommended me to use 2 uL to transform my cells...

01 January 2019 10,003 3 View

Water or kit elution buffer to elute DNA for gel extraction?

I have PCR-ed some DNA samples which I need to perform gel extraction on. These DNA samples will subsequently be used for restriction digestion and ligation over the next week. I was advised to...

18 December 2018 4,067 7 View

Molecular cloning problems?

I've been trying to clone a ~100 bp insert into a ~12000 bp vector backbone. Basically, what I've done is to do the standard things (restriction digestion, PCR cleanup/gel extraction, ligation,...

17 December 2018 959 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View