Has anyone tried to fluorescently label a Histagged protein bound to a Ni-NTA column?

We have a protein that is soluble when bound to the column but precipitates at the high protein concentrations and high pHs required for all of the coupling reactions. We were thinking of labeling...

25 August 2019 5,840 3 View

Can hearing stories support individuals and communities to create a new perception, a way of seeing that did not exist before hearing the story told?

As oral storyteller and researcher, my approach is heuristic; it is my experience that folktales and life stories offer encouragement, via the characters/protagonist journey, that fosters sound...

12 November 2017 4,938 2 View

How to compare population estimates from distance sampling performed by only one observer and two observers?

the two observers will walk together and collet the same data (sights)

16 February 2017 2,530 6 View

Are there any books or journals I should read that may provide information about how long an intervention should last in a quantitative study?

I am conducting a quantitative study using a quasi-experimental design. I need help locating information about required sample size and how long the intervention should last. Are there any...

12 March 2015 4,218 3 View

Leishmania infection in mixed C57Bl/6xBalb/c mice

I am having a hard time finding literature reports on infection of C57Bl/6xBalb/c mice with Leishmania major. If I have an F1 generation of mice from C57Bl/6 and Balb/c, and infect them with L....

20 May 2014 7,016 2 View

Is there any method that uses private haplotypes as a measure of population genetic structuring?

The traditional methods to detect population structure like Fst sometimes are not able to detect differences among locations or populations even when they exist. In a case where there seems to be...

23 January 2014 5,556 3 View

Can monoclonal antibodies be used as anti-infective agents?

Seeking antibodies directed against bacterial or viral proteins that have proof of efficacy

28 July 2013 9,013 1 View

No title

It is exposing the theoretical TPACK about integration of educational technology in the teaching/learning process, reflecting on the need for teachers to have continuous professional development.

18 June 2013 3,112 7 View

Which plasmid to use for expression of two proteins?

We are interested in expressing two proteins from the same plasmid. Which vector is the best? Is it possible to use the same promoter for both? We are not interested in bicistronic, but in a...

27 May 2013 4,189 7 View

Does anyone use Tableau Software for database analysis?

I'd like to share tips and tricks about this useful software.

16 April 2013 8,742 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View