Does who know why SDS-PAGE run like this?

samples are unaligned as picture. I guess this reason is uneven charge on machine. but i don't know precisely reason.

06 July 2018 4,137 4 View

Western blot doesn't work well, what's a problem?

Recently, I got a problem on western blot. Smaples are not apart as molecular size, and even marker is not apart from about 70kb to 130kb. I did make a new gel buffer twice, changing tris and 10%...

06 July 2018 8,629 7 View

Could i use small intestinal epithelial primary culture cell for researching colon?

I would research about colon. So i am going to do primary cell culture. when I searched "mouse colon cell primary culture", but results show me (small) intestinal epithelial cell primary culture...

04 May 2018 6,124 0 View

Is intestinal epithelial cells used for reserch of colon cell profiles?

I would do mouse colon cell primary culture. So I googled mouse colon cell primary culture. But a results show me intestinal epithelial cells. these intestinal epithelial cells are colon...

03 April 2018 9,948 3 View

Can I re-use a transfection supernatant?

I did transfect cell, and all cell died. And I did discard transfection supernatant, careless. Our lab has no more transfection virus, and other person has used transfection supernatant. But it...

02 March 2018 1,430 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View