Hello,

The amount of proteins in one or more samples might be too important and modified the migration around the sample. One sample have too much salt and locally modified the migration. Last, you may have a problem of electrode....

Best Regards

Hi there,

The simplest way to test if it's a sample or an apparatus issue is to load the same samples in a different order and see what happens.

Hi, this problems, together with Stephane suggestion, colud be do to uneven solidification of gel if they are handmade, acrylmide filament attached in the little metal wire in the low section of apparatus.

You can wash better glass and metalic wire in the apparatus.

You can exclude samples problem if you load marker or only laemmli buffer in a new gel.

You can make this test, at the same time, with another apparatus.

Hi,

I agree with the suggestions of other colleagues but i will suggest you check whether your running buffer is leaking or not because sometimes if you see dye front shows this shape,it might be due to shortage of the running buffer at the top of the gel cassette .Ensure that the gel cassette is full to the brim before loading your sample and there is no leakage.

All the best!