I would do mouse colon cell primary culture. So I googled mouse colon cell primary culture.
But a results show me intestinal epithelial cells.
these intestinal epithelial cells are colon cell?
And somebody let me teach mouse colon cell primary culutre method?
also i want to about pancreas, too.
I am not familiar with intestinal cells, but have multiple years experience with primary pancreatic acinar cells. This protocol is quite good for the isolation and characterization of primary pancreatic acini: Article Isolation of Rodent Pancreatic Acinar Cells and Acini by Col...
Thank you Minseok for your question. I used to isolate human and rat liver cells to study them during my PhD and postdoctoral fellowship, about 30 years ago. Regarding your question, I attach a book chapter that will provide details about isolation of human colonic mucosal cells. I will look for animal studies, although I believe the method described here could form the basics for your study. Best wishes, Prof S Azer
Yes, primary colon cells are intestinal epithelial cells that come from the colon as opposed to the small intestine. Whether you need a protocol that gives a single cell epithelial suspension or an organoid culture will depend on your experimental aims. This is a link to a paper with a protocol for long term culture of intestinal crypts: https://www.gastrojournal.org/article/S0016-5085(11)01108-5/fulltext
- Badges
- Science method
- Similar topics
- Teaching