Anita Tewari

3 Questions 6 Answers 0 Followers

Questions related from Anita Tewari

Which protein (other than heavy chain) my Goat anti mouse IgM (heavy chain) polyclonal HRP conjugate is picking from serum?

Hi, I am running western blot on serum for IgM and using Goat anti mouse IgM (heavy chain) polyclonal HRP conjugate as secondary Ab (Not using primary Ab, direct secondary). The secondary Ab is...

02 February 2019 4,065 3 View

Why i am getting smile bands and diffused bands in my blot?

I am using Biorad precast gels (4-20%) for my experiment for detecting IgG and IgM in the serum. For that i directly dilute my serum samples and load with 2X SB or 4X SB (13-15ul/ well) and run my...

12 December 2018 10,023 0 View

Trouble with protein ladder in western blot. what could be the reason?

I am using 4-20% biorad precast mini gel and protein ladder is also from biorad. I have attached the image of membrane. Running voltage- 100 V for 1 hr at room temperature and 70 V for 2.30 hr at...

12 December 2018 8,526 6 View