Can anyone help me to remove a hydrophobic peptide from C18 column?

I have injected a hydrophobic peptide into analytical C18 column and i have tried to cleaning the column applying a lot of protocols using acetonitrile, methanol, isopropanol, but in the blank...

05 January 2021 3,305 3 View

How can I model a prediction of a dependent variable using BOTH a predictor at time 1, time 2 AND the change (time 2-1) of this predictor?

I want to model the following: DV: Y at time 3 IVs: X at time 1, X at time 2, change of X from time 1 to time 2 Which is the most appropriate way (preferably in SPSS). I am afraid I cannot use...

10 December 2020 264 3 View

Can you recommend me tree databases?

Hi. I have slight experience in searching phylogenetic trees, normaly I use TreeBASE or Dryad but some papers that I found doesn't have the resulting trees in these databases or there are genetic...

26 October 2020 582 3 View

Could ANOVAs be used as a substitute for regression models for mediation analysis?

Hello all, I hope you can help me with these questions. I am developing a mediation analysis using the mediation package in R. I need to analyze the influence of perceived learning (Mediating...

18 August 2020 7,521 9 View

What would be the fate of ecotoxicology in the South American studies?

I'm currently working on ecotoxicology and I'd like to know what my colleagues from the same area think, especially in South America. Too much of microplastics in my opinion, but few studies...

18 May 2020 1,064 2 View

How to choose the best smooth algorithm for your data in Origin?

I have several curves of applied potential versus electric current with hundreds of points in each of them. I am trying to analyze their first and second derivatives, but they are too noisy to...

27 April 2020 5,932 2 View

(epidemiology) how does one measure the RATE of infectivity?

I am not an epidemiologist, nor am I studying epidemiology, this is a question born of sheer curiosity due to current events. I understand (more or less) the principle behind the basic...

19 March 2020 9,458 3 View

How can I clean insect exoskeleton?

I want to remove whole soft tissues and maintain intact the exoeskeleton

10 March 2020 4,345 6 View

How would you evaluate "Interpersonal Trust" for people who are sharing a 4-seat vehicle with people they do not know?

Hi. I am conducting an experiment and I would like to understand if people would be willing to share a 4-seat vehicle with people they do not know. I have thought that the personal tendency of...

10 December 2019 7,989 3 View

How to handle output data for Taguchi DOE?

I am using a L8 (2 level, 4 Factor) DOE. I have completed the experiments and am now trying to sift through the data and start the analysis. I completed these experiments in a strictly production...

03 December 2019 1,977 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View