Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Why can`t I isolate RNA from Extracellular vesicles?

Hello, I have been trying to isolate RNA from EVs (from MSCs) for a while. I isolate the EVs using SEC. Then I use the "Total Exosome RNA and Protein Isolation Kit" from Invitrogen. When I...

21 October 2020 8,559 4 View

How to directly use a directory of .h5 files as the training set in Python without appending them to a list?

I am trying to append .h5 files saved in a directory for my training set using Python, but I face memory error as the Data set is very large. The following code is what I used for...

23 April 2020 9,649 2 View

To record trajectory tracking performance of a controller, is it resonable to calculate the mean_square_error or the normalized-measn-square-error?

I some papers, the mean square error is considered. In some other, the mse is normalized by dividing the error by the (total sampling instants x the total length of the reference...

25 February 2020 784 4 View

What is the most reliable guide for identification of alternaria species based on morphological and cultural criteria?

what is the most reliable guide for identification of alternaria species based on morphological and cultural criteria?

02 December 2019 174 1 View

How the nonholonomy of mobile robots allow control of the system with less control inputs?

In "Simultaneous Stabilization and Tracking of Nonholonomic Mobile Robots: A Lyapunov-Based Approach", the authors stated that the nonholonomy of mobile robots allows control of the system with...

27 May 2019 7,721 4 View

How to interpret DMA?

18 May 2019 631 6 View

What are the most reliable tools or softwares for designing of the CRISPR gRNAs?

Dear all, Which tool is more reliable? CHOP CHOP or CRISPRdirect? I need some information about other tools and softwares. Thanks a lot

08 May 2019 10,004 2 View

If you could recommend one significant change to make Higher education more affordable without compromising its worth, what would it be?

Higher Education cost is increasing and students encounter some challenges to afford it.

21 March 2019 7,173 6 View

How to match reflection in both visible and infrared?

I have a thin film of a fluorescent material and want to make a reference sample with similar reflection in both visible and infrared to do the comparison. How can I make the reference sample?

26 February 2019 6,857 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

What are effects of steel jacketing on flexural strength?

Dear all, If steel jacketing is used to retrofit a reinforced-concrete (RC) column, can we use it to improve flexural strength? I understand that we leave gaps between the ends of the jacket and...

22 February 2021 1,906 5 View

How to model steel jackets using SeismoStruct?

Hi all, Does SeismoStruct support modeling rectangular RC columns retrofitted with steel jackets (e.g. rectangular, elliptical) ? Any suggestions for other software (apart from...

20 February 2021 4,521 1 View