Commercial materials/surfaces for contact measurement?

Does anyone know of, or use, commercially produced surfaces for contact measurements (control material)? As an example, is there a producer of PTFE discs with highly controlled surface roughness...

09 April 2020 8,806 5 View

What are the pros and cons of running virtual conferences?

What are the pros and cons of running virtual conferences? I ask this question because the current challenging situation with the COVID-19 pandemic has, understandably, meant the cancellation or...

27 March 2020 8,346 17 View

Why is 'Scale' considered a spatial continuum, but not a dimension of space?

Consider the books 'Cosmic View; The Universe in 40 Jumps' by Kees Boeke (1957 - the original inspiration for the others), 'Sizing Up the Universe: The Cosmos in Perspective' by Gott, J. Richard...

27 December 2019 1,030 1 View

How to calculate photovoltaic power output from solar radiation for 100 sites countrywide?

I would like to calculate PV output for 100 sites for one year using hourly solar irradiation and temperature data. Can anyone suggest how I can use a software (e.g. PVSyst, RETScreen or similar)...

21 October 2019 1,061 3 View

Which statistical test for repeated measures ANOVA with multiple patients and biological replicates?

I am analysing human neuronal samples in culture after treatment with different concentrations of NGF. For each patient I am analysing around 30-40 neurons for each of 4 concentrations (including...

05 June 2019 6,162 2 View

Why does my protein destain so quickly?

Hello all, The protein I'm purifying seems to destain very quickly on Coomassie stained SDS-PAGE gels (red box in image). I've heard that proline-rich proteins (like this protein) have a...

06 March 2019 173 6 View

Systemic/social constructionist thinking about trauma?

I'm writing about trauma from a systemic/social constructionist perspective, and wanting to bring in ideas around the 'power threat, meaning framework' that challenge traditional bio-medical...

18 December 2018 2,235 7 View

DAPI and Hoechst are not staining brightly enough to detect, what other nuclear stain can I use with the Sigma TRAP leukocyte stain kit?

08 September 2016 5,393 3 View

DNA in gram quantities? Where can I purchase/obtain it?

Does anyone know where to obtain pure, 1-10 gram quantities of DNA? Lots of PCRs, or perhaps just bulk DNA purification from cells come to mind, but I was hoping to get pure, controlled sequences...

05 June 2016 7,536 3 View

Does anyone know of literature/guidelines regarding sample sizes when research different components of the same treatment?

I was listening to a presentation by prominent psychotherapist/researcher awhile back and the presenter mentioned that when doing comparative effectiveness research on different components of the...

05 January 2016 2,743 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View