"WaitFlag Error" in SR785 Dynamic Signal Analyzer followed by automatic reboot?

My SR785 Dynamic Signal Analyzer keeps rebooting after showing a "WaitFlag Error" in between measurements. I have been unable to find any mention of this error in the service/user manuals...

23 December 2020 8,427 2 View

Is there a solvent for polypropylene at room temperature?

I want to dissolve polypropylene for making a solution so as to make fibres at room temperature. I have tried toluene and xylene but without any success in dissolving.

06 December 2020 8,479 2 View

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I have tried using PowerSoil Pro kit from Qiagen with slight modifications- incubating at 65 degree for 10 mins after adding solution CD1, increasing the number of washes with CD5 to 3 times and...

27 October 2020 7,782 3 View

Can two analyses from the same study (two different time points) be used within the same meta-analysis outcome?

I have a study which reports PTSD diagnostic status at both 3 and 6 months. In my meta-analysis of the intervention, both 3 and 6-month assessments fall within the same outcome assessment (for...

26 March 2020 2,432 3 View

Indicator variable - Pooled regression results vs data partition results?

This is the Base regression: Y = b0*X1+b1*X2+error term I am using an indicator variable Z which takes value 1 for years 4 to 6 and value 0 for years 1 to 3. I am trying to analyse the impact of...

05 March 2020 9,865 3 View

Using islets immediately upon isolation?

Hello. Our incubator for primary cultures is broken but I need to isolate some islets for both qPCR and estimating the insulin content. Has anyone performed either of these techniques on freshly...

18 November 2019 1,352 3 View

Protein model (I-TASSER-Ramachandran plot) docking ?

Protein on which i am working is a disordered protein and no crystal structure of this is available on PDB. I have modelled structure using I-TASSER. Out of top five models generated by I-TASSER I...

11 August 2019 9,604 5 View

How to combine treatment arms for dichotomous variables?

Hi, In my meta-analysis, the intervention is often compared to more than 1 control group. I am analysing a dichotomous variable (PTSD diagnosis). With continuous outcomes (such as symptom...

30 April 2019 8,168 2 View

How to convert commercial microwave oven intro sintering furnace?

I'm having manumaster comeercial microwave oven with 1.1kW power and 2.45 GHz Frequency. I want to convert it into sintering furnace and for the same i have managed tooling requirements like...

25 April 2019 8,401 3 View

Can we perform swelling ratio studies on lyophilized hydrogel powders? If yes, how?

Or it can be done only after drying the hydrogels (gravimetric method)

24 October 2018 5,870 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View