8 Questions 12 Answers 0 Followers
Questions related from Shruti Bagla
I routinely use Mutation Taster to predict functional impact of a mutation in silico. However the input on MT is either ORF position, cDNA (transcript: ORF with UTRs) or genomic location on the...
11 July 2019 3,464 3 View
Our lab outsourced whole exome sequencing to a company, and I received the analyzed files recently. I have always been a wet lab person, and do not understand most of the file types, and how to...
30 March 2018 5,807 5 View
I have two mutations(of interest) on a gene, one of which is in intron (splicing region) and another coding sequence. i want to check two things: whether the intronic mutation is resulting in a...
07 August 2017 2,683 3 View
We are interested in looking at vascularization and angiogenesis in some human brain samples. The ideal way would be to use immunostaining but our lab is not developed for IHC or any...
20 June 2016 579 1 View
I want to study the effect of gene knockdown on differentiation. If I plate the cells for 4 hours, before adding the siRNA or S-TuD, and then add fresh media with retinoic acid to induce...
18 August 2015 1,423 3 View
I want to design an experiment where we treat the neuroblastoma with quinolinic acid. Has anyone done this? Or treatment of primary cultures with QA? Where do you buy QA? And how do you suspend...
08 May 2015 6,959 2 View
I am trying to knock down a gene of interest using siRNA in SH-SY5Y cells. But the amount of siRNA I use is mostly based on the well surface area etc. I use 50pmol/well in a 6-well plate. So is it...
18 March 2015 4,630 3 View
Hi, We have used FuGENE 6 (Promega) to transfect Sy5Y cells with plasmid DNA, but I just spoke to the promega tech support and they do not recommend FuGENE products or any of their transfection...
04 November 2014 4,547 2 View
