Any specific method to get PCR amplifiable DNA from Padina sps?

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No title

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Alejandro Martin

Use Google/Pubmed to find out whether other researchers have had the same problem. Perhaps there is a known Taq Pol inhibitor in DNA preparations from that species? In that case, do serial dilutions of your sample and try them by PCR, to find out whether you can find a sweet spot at which the inhibitor is sufficiently diluted but the DNA is still amplifiable.

Susantha Udagedara

Please refer flowing links, they mention about methodology

https://www.google.lk/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&ved=0CC4QFjAC&url=http%3A%2F%2Fwww.researchgate.net%2Fpublication%2F227562500_An_effective_DNA_extraction_protocol_for_brown_algae%2Ffile%2F9fcfd50f616d6453bd.pdf&ei=Y7_pUraKCaSMiQf3noHICg&usg=AFQjCNG1tn6oRGRKlUrvpu4H6TA5mc195Q

https://www.google.lk/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&ved=0CDcQFjAD&url=http%3A%2F%2Fwww.vliz.be%2Fimisdocs%2Fpublications%2F98632.pdf&ei=Y7_pUraKCaSMiQf3noHICg&usg=AFQjCNFM4u5l9QcJKPdtxAtOzQkaiRKZyQ

http://www.oceannet.org/library/publications/documents/marine_samples.pdf

http://meacr.org/2ndACPrgnAbs.pdf

http://www.carsoncenter.uni-muenchen.de/download/publications/perspectives/2012_perspectives/1203_sickness_web_color.pdf

William D. Robbins

I found amplifications I did on shark tissue to be extremely sensitive to magnesium chloride concentrations. No idea if this will apply to Padina samples, but worth considering if the other suggestions don't help!