How to detect TGFb and IL10 from activated Tregs?

Dear colleagues! I plan to check cytokine production by my WT and KO Tregs following activation, particularly, IL10 and TGFb (TGFb1 or LAP, exactly) as these are reported to be the major...

02 December 2020 9,424 5 View

Why CD4 Treg lose FoxP3 during expansion in vitro?

Hello everyone! I have the following question: I tried to expand CD4 Tregs in vitro to get enough material for WB or any other application. This is my first trial with them. So, I`ve got FoxP3+...

13 September 2020 6,458 6 View

How should I care of mice after sublethal irradiation?

Hello dear colleagues I plan to test the primary antiviral response of CD8 T-cells of different genotypes, WT and with total knockout of some gene. The problem is that the KO mice that I plan to...

22 July 2019 1,844 3 View

Do exist any approved to human (or, at least, near to be) anti-inflammatory drugs, alternatives to NSAIDs?

09 August 2017 2,919 7 View

Classifier robust against non-informative features?

I have two feature sets I evaluated using SVM on a two-class problem. The first set (the "good" one) provides acceptable results, while the second one is far behind. When I merge the two sets...

12 March 2014 161 3 View

How do you specify CPSET and NSET for history output?

The simulation, I am trying to bring about, is essentially related to a contact analysis between deformable and analytical rigid bodies, the interaction between them is defined by means of surface...

13 February 2014 1,142 1 View

Dear colleagues! Do you think that all the team roles according to the Belbin model* will remain unchanged in the future?

Perhaps, some will cease to be relevant and, conversely, new ones will arise? And if the changes are still there, then what and why? (*Belbin model =...

01 January 1970 9,973 4 View

Transition from academic to business career: mission impossible?

Dear Colleagues! As far as, from your point of view, it is possible (and justified) to implement the concept of Life Long Learning in a professional academic career by completely withdrawing from...

01 January 1970 4,724 15 View

The need to disseminate information about the results of their scientific activities for the researcher in modern conditions.

Dear Colleagues! Do you think a modern researcher should independently make efforts to spread awareness in the world around him about the results of his scientific activity? If so, how much will...

01 January 1970 9,054 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Is it Ok to decline to write a chapter for a IntechOpen to the lack of experience?

I have been asked by IntechOpen to write the chapter for a book they are going to publish, but as I don't have enough experience in the area they proposed me to write I declined their offer. Now...

24 February 2021 2,802 4 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

Which DL architecture is better to work on handwritten data?

I am working in the area of Handwriting recognition for Bangla. As there are many DL architecture, I am confused which one to select.

15 February 2021 4,996 1 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View