I have used two different BDNF antibodies in the past with mixed results:

- AB1779SP (Millipore; 1:500) works OK for WB detecting the monomer at 18KDa and homodimer at 27-28KDa, with some additional unspecific bands (you may want to use a higher dilution). In IHC seems to work fine in fixed sections from hippocampal slices, although I only tried once.

- sc-546 (Santa Cruz; 1:200) works well on WB but haven't used it for IHC.

You can check Fig. 2E in this paper to have an idea how it looks on WB: http://jcb.rupress.org/content/210/7/1225.long

Hope this helps!

Victor

Thanks a lot Victor for the detailed answer!

Will look at them now.

If you do not have luck with the commercial antibodies, Amgen has an antibody that works beautifully at very low concentrations (rabbit anti-BDNF

antibody @ 1:6000 ). Sometimes they will donate antibodies for research (particularly in the case when the commercial antibodies are not as effective).

Thanks a lot Andrea!

So far I have tried 2 different BDNF antibodies,

1) anti rabbit BDNF (Gene Tex): it seems this antibody could not detect monomer form of BDNF

2) anti rabbit BDNF( Santa-Cruz): It works well and detect the monomer form but sometimes the band is not good looking or wavy

Thanks a lot Sina! This is very informative.

Has anybody observed 20-22 kdA BDNF band along with 14kda mBDNF, 28 kDA truncated BDNF and 32 kDA Pro-BDNF? What is this 20-22 kDA BDNF band?