7 Questions 35 Answers 0 Followers
Questions related from Apurva Agrawal
Hi, I am trying to look for basal ERK phosphorylation in N2a cells. Although, I am able to detect very good intensity of bands for total ERK, but the p-ERK bands are hardly visible. Also, when...
12 December 2017 4,847 5 View
I recently performed some experiments where one of the stimulants elicited calcium oscillations in cultured astrocytes. However, even though all of them are oscillatory, the oscillation pattern of...
05 May 2014 8,197 2 View
I am working on a protein which becomes active when phosphorylated at a particular tyrosine site. I am looking for methods/ strategies as how to make constitutively active mutant for this protein....
10 October 2013 7,642 16 View
I am looking for specific literature to understand what happens with the calcium levels during early LTP phase and Late- LTP phase. It is very well known Calcium influx is necessary for LTP...
01 January 2013 1,301 1 View
01 January 2013 6,303 2 View
I am studying a protein which has a DNA binding domain. I am looking for a bioinformatic tool which can predict in which regions of DNA my protein of interest can bind. I want the putative DNA...
12 December 2012 9,304 2 View
I am currently working on preparing amyloid beta (1-42) oligomers and characterising them. The protocol that I use for the same is: 1. 1mg of amyloid pure peptide is dissolved in HFIP (400 ul)...
11 November 2012 9,273 54 View
