08 August 2016 3 6K Report

Hello,

I am interested in quantifying the number of cells undergoing apoptosis in the brain of 1 month-old mouse model for a neurodevelopmental disorder. These mice were perfused with PBS and 4% PFA, and were left in 4% PFA overnight. Then they were cut to 100um coronal sections and stored at 4c.

These mice were not treated with any apoptosis-inducing treatment.

I understand that Roche has a good in situ apoptosis detection kit, but I am not certain which one exactly is the relevant one for me. Could you please advise? If possible, could you refer me to a specific product you recommend?

Thanks

Boaz

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