I'm looking for effective transfection reagent for siRNA treatment of C6/36 cells. I've used DharmaFect and Lipofectamine2000, but it gave transient knock-down of the protein or ineffective knock-down.
E.g. X-tremeGene HP DNA transfection reagent (Roche, USA)
(For ref. see PLoS Negl Trop Dis. 2015 Jul 1;9(7):e0003885. doi: 10.1371/journal.pntd.0003885.)
You can try shRNA knockdown, or genome editing to knock out. Sometimes, double siRNA transfection may increase the knockdown efficiency.
Fakhriedzwan Hj Idris
When starting any transfection experiment, I recommend optimizing first with a GFP plasmid to check transfection efficiencies. Once you have optimized that, then you move onto siRNA. And most people recommend using a pool of 2 or 3 siRNA for optimal knockdown.
Thank you for all the recommendations. I really appreciate it
RNAiMax reagent works great for delivery of siRNA into a wide range of cells. for tough ones consider electroporation- Neon or other system
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