8 Questions 13 Answers 0 Followers
Questions related from Wensheng Xie
I need to treat cells with retinol. Does anyone know what is the best way to prepare retinol stock solution, and how to prepare the work solution? How to store the stock? Can it be re-used? Thanks!
30 August 2021 1,502 1 View
Cells will have lower viability upon treatment of high concentration of fatty acids, particularly with Palmitic acid. Does anyone know any sensitive biomarkers to detect the lipotoxicity process?
03 January 2021 9,975 1 View
What is the mechanism of CD3 Ab alone to activate T cells? It is different from antigen dependent activation which requires MHC molecules. It seems these are separated mechamisms, but also closely...
03 August 2018 4,787 4 View
I would like to know information about esiRNA form Sigma for the following points: 1. transfection protocol; 2. knockdown efficiency; 3. off target effects; 4. toxicity. Anyone would like to share...
01 June 2018 9,796 2 View
I used a validated antibody to pull down a target. After IP, I was able to see strong bands in the right size of the target protein, and also very strong signals in around 150KDa with Ab agains...
09 February 2018 7,425 4 View
I need to perform IP for a target which binds to lipid droplets. I used RIPA to prepare cell lysates for western blot which showed good results. But not sure whether it is good for IP.
18 January 2018 3,447 6 View
I would like to compare the features and pros/cons between antisense oligonucleotides and siRNA in oligonucleotide therapeutics. Both can be used to inhibit target gene expression with different...
01 January 1970 7,226 2 View
Any information or any publication about the profiles with these different stimulation methods? such as cell surface markers, cytokines, proliferation rates, cell types stimulated ... I am more...
01 January 1970 3,055 4 View
