Wensheng Xie

9 Questions 13 Answers 0 Followers

Questions related from Wensheng Xie

How to prepare retinol solution for cell treatment?

I need to treat cells with retinol. Does anyone know what is the best way to prepare retinol stock solution, and how to prepare the work solution? How to store the stock? Can it be re-used? Thanks!

30 August 2021 1,506 1 View

What biomarkers for lipotoxicity detection?

Cells will have lower viability upon treatment of high concentration of fatty acids, particularly with Palmitic acid. Does anyone know any sensitive biomarkers to detect the lipotoxicity process?

03 January 2021 9,983 1 View

How would CD3 Ab activate T cells in the absence of MHC? Which type of T cells would it mostly activate?

What is the mechanism of CD3 Ab alone to activate T cells? It is different from antigen dependent activation which requires MHC molecules. It seems these are separated mechamisms, but also closely...

03 August 2018 4,791 4 View

How does esiRNA from Sigma compare to Dharmacon's SMARTpool siRNA?

I would like to know information about esiRNA form Sigma for the following points: 1. transfection protocol; 2. knockdown efficiency; 3. off target effects; 4. toxicity. Anyone would like to share...

01 June 2018 9,798 2 View

Is my target protein ubiquitinated?

I used a validated antibody to pull down a target. After IP, I was able to see strong bands in the right size of the target protein, and also very strong signals in around 150KDa with Ab agains...

09 February 2018 7,433 4 View

Is RIPA buffer good for IP?

I need to perform IP for a target which binds to lipid droplets. I used RIPA to prepare cell lysates for western blot which showed good results. But not sure whether it is good for IP.

18 January 2018 3,450 6 View

Antisense vs. siRNA

I would like to compare the features and pros/cons between antisense oligonucleotides and siRNA in oligonucleotide therapeutics. Both can be used to inhibit target gene expression with different...

01 January 1970 7,234 2 View

There are several ways to stimulate T cells, such as using PHA, anti CD3 CD28 Ab or beads, also anti CD2CD3CD28 beads. Any outcome differences?

Any information or any publication about the profiles with these different stimulation methods? such as cell surface markers, cytokines, proliferation rates, cell types stimulated ... I am more...

01 January 1970 3,057 4 View

Metabolic network among free fatty acids, DAGs, TAGs, PCs

I would like to have some discussion about lipid metabolism involving free fatty acids, DAGs, TAGs, PCs, PEs. In particular, how do TAGs, PCs and PEs get connected in the metabolic network?

01 January 1970 3,455 0 View