Fakhriedzwan Hj Idris

5 Questions 8 Answers 0 Followers

Questions related from Fakhriedzwan Hj Idris

How long can cleaved caspase 3 be detected during infection in vivo?

I am investigating the apoptosis of T cells during dengue infection by detecting the cleaved caspase 3 in purified T cells isolated from infected spleen. I harvested the spleen 5 days...

23 April 2021 7,821 2 View

Any recommendation on transfection reagent for siRNA treatment of C6/36 cells?

I'm looking for effective transfection reagent for siRNA treatment of C6/36 cells. I've used DharmaFect and Lipofectamine2000, but it gave transient knock-down of the protein or ineffective...

05 November 2019 5,219 5 View

What is the best method to sterilize lysates which potentially contain peptide?

I'm doing a screening on lysates with unknown properties against virus on cell culture. I need to make sure the lysates are sterile so as not to contaminate my cell cultures. What is the best...

23 November 2015 4,396 3 View

Will expired media have a major effect on cell growth?

I am working on cells requiring EMEM as the growth media, however, the batch that I have are expiring soon. I want to know if this will have a major effect on the cells in terms of their growth or...

23 October 2015 9,679 2 View

Will there be a competitive binding between secondary antibodies in an immunofluorescence study of virus-receptor interaction ?

I am planning to do a virus-receptor interaction study on murine cell lines using immunofluorescence technique. I am planning to use rabbit anti-DENV Abs and rabbit anti-PDI abs as my primary...

13 April 2015 8,182 6 View