Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

What is the best way to prepare anaerobic medium?

Hi, I would like to prepare anaerobic medium for cultivation of gut microflora. I have an anaerobic chamber. How should I remove oxygen from the medium? Is it enough to leave it for 48 hours in...

05 October 2020 3,093 4 View

Is it possible to do SEM on a cross-section cut of a fresh leaf?

Hello, I'm working with pulsed electric field applied to lemongrass leaves to generate pores on the cell membrane to enhance essential oil extraction. I would like to know if there is a way to...

20 October 2019 1,364 3 View

How can I preserve leaf samples to measure the trichome density?

Hi. I'm working in a tropical rainforest and I need to measure the density of trichomes, but due the lack of equipment in the station, I need to preserve the samples during 1 month to then count...

10 October 2019 1,460 4 View

Intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

Hi! Can anyone suggest a protocol for intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

19 March 2019 9,262 3 View

Any experience/opinion on sociolinguistic interviews without the interviewer, i.e., only a questionnaire and the interviewees recording themselves?

I want to interview non-native speakers of English, but I am not a native speaker, so to avoid certain implications of this (unnatural setting, accommodation, etc.), I am thinking of sending them...

11 October 2017 4,867 4 View

Does anyone know of a good reference that describes the biology of monocyte/macrophage recruitment and differentiation during wound healing?

Please send me the title/author if you know of a good reference. Thank you!

04 October 2017 7,695 3 View

How do I do immunocytochemistry staining on non-adherent cells?

Are centrifugations required between every wash step/blocking/antibody incubation in order to not lose the cells? Is there a way to make them adhere through a fixative or some other process in...

03 October 2017 8,611 4 View

Staining plant by Safranine O Fast Green FCF?

14 March 2017 1,404 1 View

Does anyone get a high background fluorescence when imaging cells stained with CellRox Red? Any suggestions on how to reduce?

I am staining primary macrophages in complete media. There is a high background when imaging the media controls as well as the positive control (400uM TBHP) Any suggestion on how to reduce? Thank you!

30 January 2017 6,000 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View