Could you please advise me that any suitable techniques for this experiment which will be done shortly?

I am planning to perform the functional study of gene whuch is supplied maternally, so morpholino-mediated knockdown assay won't work. Also about the CRISPR mediated knockout study need to wait...

27 December 2020 731 1 View

How do we apply a bending stiffness on to a wire model in abaqus?

I was trying to mimic the deflection of a cantilever beam in wire on to a model with a portion of the beam replaced by a spring which provides a bending stiffness. I gave a usual spring with...

01 July 2020 6,869 4 View

How to rectify the"DIsplacement Increment for contact is too big" warning in abaqus?

I am currently modelling a joint of a geodesic dome. I previously ran an analysis only with preload of bolts ignoring all contact surfaces of the shank of the bolt and other geometry . This time...

03 June 2019 4,811 5 View

How to generate adsorption isotherms ?

We are doing a project on adsorption of hexavalent chromium using agricultural wastes. I would want to generate adsorption isotherms for our data. Would I be able to know the procedure for the...

24 May 2019 1,655 13 View

Which is the good and required structure that need to be designed to solve current problems in a society?

I am a final year UG student, this semester we need to do design project. But we have learnt only member design in our curriculum, so I am looking for a structure by designing that we can get a...

15 June 2018 1,986 7 View

How can I address the issue of a significant change in the Control group?

A study on effect of a particular yogic maneuver was carried out in our department. It involved two groups, control and intervention group( n = 50 in each). It so happened that, at the end of...

26 April 2015 4,409 3 View

What are the alternate materials for machine bases other than cast Iron ?

Material science are developing to great extent kindly share the information on alternative material for cast iron. I came to know that some of the machine bases are made of polymer concrete,...

04 February 2015 456 13 View

Which is the best element choice for muliti layered FRP material in ANSYS ?

I have to conduct FE analysis of aircraft structure of GFRP material which is of multi layered kindly suggest me how to do modelling of multi layered elements with different material properties in...

18 December 2014 9,482 4 View

What is the equivalent concentration of lactose to induce T7 based recombinant expression instead of IPTG?

Hi Guys, Lactose is a metabolisable sugar molecule and affected by inducer exclusion and catabolite repression. Any experience of using lactose as inducer instead of IPTG to improve recombinant...

10 November 2014 4,662 3 View

How will co efficient of friction change with respect to the change in contact area ?

Does the co efficient of friction depend only on materials in contact? In frictional clamps the co coefficient of friction plays a major role, as co efficient of friction directly depends on the...

09 October 2014 1,469 19 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

LC-MS metabolomics data after annotation some have their compound name but no KEGG id or with KEGG id but no pathway. How to interprete?

Recently I have generated LC-MS metabolomics data. After annotation, some have their compound name but either no KEGG id or KEGG id but no pathway. How to interpret those compounds? Additionally,...

18 January 2021 1,938 3 View

How can I build a local KEGG db?

I want the info (gene name, metabolic pathway, and organism of origin) of some key identified metatranscriptome genes obtained from MG Rast (I have the sequences). The KEGG ftp site seems to...

12 January 2021 9,985 1 View

Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Analysis of significance by using R programming ?

Dear All, I have the data of metabolic pathways in case and control, well there are pathways which are represented several times due to the involvement of reactions in case, well now I would...

09 December 2020 4,494 5 View

Can xylose be formed from fructose over acid catalysts?

I am new in the field of sugar chemistry. I wanted to know if it is possible for fructose to be converted to xylose over acidic conditions. I saw in some schemes a pathway from fructose to xylose...

07 December 2020 5,247 3 View

Why do cell sensitivity to antibiotic resistance increase after lentiviral infection?

03 November 2020 7,754 2 View

What is the difference between endometriosis and ovarian cancer in terms of signaling pathway?

Hello Recently, I am curious to know about mechanisms of endometriosis and signaling pathways. I have a question about the difference between signaling pathways in benign tumors and malignant...

26 October 2020 3,891 6 View

How to construct Scatter plot for KEGG or GO classification visualization?

Can anybody tell me how to construct this scatter plot for KEGG or GO classification like the image I have attached?

27 September 2020 5,359 3 View