Any one interested in working on organogenesis ?

More Mansi Negi's questions See All

Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

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What are the common mistakes occur during DNA isolation in plants?

General mistakes which could happen during DNA isolation of plants

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If I have 1000 or more plant extracts, which is the best latest technology for the rapid and highly accurate identification of secondary metabolites?

the query centers on determining the most efficient and precise latest technology available in the market, capable of swiftly analyzing complex mixtures while maintaining a high level of...

26 May 2024 2,898 6 View

I have made a natural product loaded nanogels. How can I use it further for the treatment of cell lines?

I have made a natural product loaded nanogels. Through MTT assay I have found out the drug dose but am not getting how to treat the cell further with the formulations

04 March 2024 5,389 1 View

How to plot 3D Free Energy Landscapes from MD simulations with Gromacs?

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Which specific quality of flour should be targeted in order to prepare gluten free bread fulfilling all the standards ?

Quality of gluten free flour

17 August 2023 229 0 View

I'm working with a high mol. wt protein "ATM" involved in DNA repair. However, I'm unable to see ATM band in western blot in glioma patient samples?

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Enzyme is losing activity after 24 hours. what could be the reason?

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How to generate topology file for ligand in Gromacs?

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Why is Beta glucosidase enzyme is losing activity after urea treatment?

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How is the level of somaclonal variation: indirect organogenesis VS indirect somatic embryogenesis?

Does anyone know of publications where differences in the level of somaclonal variation of organogenesis and indirect somatic embryogenesis are reported, leaving aside ploidy and chimeras? It is...

20 September 2020 4,230 4 View

How Genetics approaches will help improve efficient shoot organogenesis in meristem of crop? How the experiment could be conducted?

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What are the criteria for determining subculturing time in micropropagation?

When determining subculturing time, what markers such as whether the explant shoots are green, callus size, etc.?

26 September 2019 9,335 9 View

How to choose properly adjustment factors for PDE calculation?

I try to calculate PDE for cefdinir based on the results of reproduction and developmental toxicity studies as in the attachment. According to EMA guideline, the equation used to calculate PDE...

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Phalaenopsis micropropagation in tissue culture medium?

With best wishes for all researchers i have a question about micro propagation or direct organogenesis of phalaenopsis micro propagation in tissue culture medium. I cultured the whole plant with...

14 April 2018 6,756 9 View

How time take micropropagation of saffron?

I want to try micropropagation of saffron and I search for the best method ; Organogenesis or Somatic Embryogenesis. and how time take each method ?

09 March 2018 1,219 6 View

Whitish, Compact non-embryogenic callus?

Hello everyone, I would like to know, anyone is working on somatic embryogenesis/ callus induction / organogenesis ? i need some suggestions and some articles related to the reasons behind...

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Can there be somaclonal variation in direct organogenesis?

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05 August 2017 2,061 4 View

I am working on indirect organogenesis, but fails to develop roots from microshoots so any one can suggest me, so that i'll get roots inmicroshoots?

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08 May 2017 6,054 5 View

I would be very grateful if some body has done some work on salinity tolerance of Ziziphus trees in vitro?

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Reyhaneh Sadeghi

Hi Mansi

Your question is very interesting,Do you have a specific topic?

Mansi Negi

The topic is how we can make an organ just with the help of stem cells or adult stem cell. many people suffer from the graft rejection problem. what if we are able to develop an organ from the given data base of that person which will be exactly the same.

Thank you.