How can sperms protected from the acidic PH of the vagina?

Discuss the means by which sperms can be protected from the acidic environment of the vagina?

01 February 2019 6,179 1 View

I'm so thankful to your explain for a such colleague weekly share in heavy state at RG. As, with those of a little w/share but have jump in RG score?

I will be very thankful to know your explanations about a such colleague weekly participate in heavily status at RG. In compare, with those of a little or slight weekly participate, but they have...

08 September 2018 4,284 12 View

What is your opinion of world's accelerated events and whether it has an impact on the academic and research situation around the world in general?

Is there impact on the academia and research situation due to the acceleration events around the world's now a days?. Thank you for share advance opinion.

07 August 2018 9,422 18 View

Anyone can give the meaning or help in the physiological definition of the Osmole?

04 May 2017 3,190 2 View

Whats happened to the GFR and to the renal plasma flow if the efferent arterioles constrict?

About the increase and decrease.

04 May 2017 5,499 4 View

From a male perspective. Is there any physiological differences between the castration and the vasectomy?

03 April 2017 4,595 0 View

Dear all colleagues, what is the site of secretion and the physiological functions of Von Willbrand factor ?

02 March 2017 7,843 1 View

I asked about the normal physiological values of PCV in; men, women, children, baby, infant, and at which factors it can be affected ?

About the normal PCV values ?

02 March 2017 2,169 2 View

Explain the sources of water gain?

Mention the sources of body water gaining?

02 March 2017 8,839 1 View

What are the two factors that responsible for preventing the filtration of proteins by filtration membrane?

02 March 2017 8,757 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What cause the beating of this signal?

I am trying to simulate the impact of a sinusoidal force on a Beam, yet I registred this beating signal (Displacement by time). The freaquency of excitation was the same as the first natural...

19 February 2021 5,872 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

During an anti-His capture ELISA has anyone had issues with histidine residues from secondary antibodies non-specifically binding?

Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...

07 February 2021 7,101 1 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View

Does Abaqus plugin AM-Modeler predict the same temperature/crystallinity history as UMATHT user-subroutine?

During AM process, progressively activation of elements can predict the temperature/crystallinity history, including surroundings heat losses and than this data could be used to determine the...

06 February 2021 4,686 1 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View