Hi,
I OCT embedded some cerebral organoids but am having issues sectioning them. Is there any way to take them out of the OCT and do a paraffin embedding to get thinner sections that are more well supported and give better structure?
Thank you
Ismael Hernández Núñez
I think you can just unfreeze the OCT block and take the organoid into ethanol for some days, since you have to dehydrate it before putting in paraffin. Before paraffin, you have also a step in butanol (ON) or xylol (15 minutes) to make the tissue harder. Then, you can put in paraffin for some days (it can stay for long time without problem). Finally, you can do the paraffin block.
I hope it works for you.
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