In a previous field season I collected fecal samples from nestling birds and froze them at -20 in glass vials. I have had difficulty extracting the DNA (very low yields of poor quality DNA) and I am considering trying another storage method this season. From reviewing the literature I have found:
- store at -20 in vials (as I did)
- store at -80 in vials
- store in ethanol and freeze (at -20)
- freeze in liquid nitrogen
I would very much appreciate any insights on the efficacy of each of these methods. Thank you.
Chinaza Godswill Awuchi
Check https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.4787
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Chinaza Godswill Awuchi
Also check https://www.researchgate.net/publication/329858570_From_feces_to_data_A_metabarcoding_method_for_analyzing_consumed_and_available_prey_in_a_bird-insect_food_web
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