Most recent, reliable, optimized methods to extract both RNA and protein from flash-frozen brain tissue?

I have flash-frozen brains and am looking to extract RNA for RNA-seq, and protein for western blotting. I've seen other questions related to this...

10 December 2020 6,294 0 View

How do I get started with a Research Action Project for my Capstone course?

I am totally confused after beginning this course a week ago. However, I want to fully understand so that I can make the best grade possible. I have considered topics dealing with Bullying,...

13 January 2019 5,876 5 View

Why do I get similar RNA concentrations from different amounts of cells eluted in the same amount of liquid?

I'm using the Ambion RNAqueous Micro Kit to extract RNA from cells in different pathways. I use laser capture microdissection to extract the cells. From one pathway, I normally collect 8-50 cells....

07 March 2017 8,379 4 View

Photobleaching of GFP-labeled protein when live cell imaging . can anyone help?

When we image cells transfected with live celllight-GFP proteins we notice a photobleaching when we stimulate cells with ATP but not other proteins like EGF. Can anyone help!

07 August 2014 6,033 10 View

Photobleaching of GFP-labeled protein when live cell imaging . can anyone help?

When we image cells transfected with live celllight-GFP proteins we notice a photobleaching when we stimulate cells with ATP but not other proteins like EGF. Can anyone help!

05 August 2014 6,429 9 View

Can anyone comment on the prevalence of inappropriate committee group dynamics among educators?

30 July 2014 6,751 7 View

Is there an international need for manual structural volume analysis in MRI studies?

In my research I have analysed structural volumes in MRI scans by manual tracing measures and correlated against automated measures, with particular interest in subcortical grey matter structures....

04 March 2014 2,376 8 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View