How do you know your SLICE culture is growing when you dont have any reporter gene?

Dear Colleagues, I have started growing SLICE Culture in our laboratory. I dont have any reporter gene cloned, I would like to ask, how do I make sure that the culture is growing and the cells in...

02 March 2019 8,572 2 View

Is it possible to study in vitro myelination in two chamber system?

So far I see people do Co culture to study in vitro myelination assay. It will be helpful for me if we can keep the cells separately and still do the assay. Thanks a lot.

31 December 2018 3,603 2 View

When do the transduced cells reverted back to S1/P1 if at al?

We are working on virapower with plenti6 from invitrogen from company manual they say its really one of the safest formats of lenti viral mediated gene delivery. Do we have any published document...

01 February 2018 5,502 1 View

Which one is the most reliable pig genome database?

11 December 2016 2,711 1 View

Maturation of OPC to Oligodendrocytes?

Dear researchers, I am facing problem in maturating my OPCs into mature oligodendrocytes.. I have been using T3 and T4, also adding NAC and CNTF in the maturation media, even after 2 weeks I dont...

09 October 2016 6,786 2 View

What is the most reliable method for culturing primary glial cells?

I have started isolating and culturing primary glial cells from pig. So far it has been working fine, but After certain passage of OPC my cells, as it looks like, not responding to differentiating...

06 July 2016 4,615 0 View

Primary cultures of brain cells from higher organisms?

I like to establish primary cultures (mainly neurons, oligodendrocytes and astrocytes) from brain of higher organisms.. I like to ask the experienced people what is the easiest way and simplest...

02 March 2016 4,454 2 View

What is a suitable source of human oligodendrocytes?

I am interested to start working on human oligodendrocytes. My colleagues had purchased it from ATCC and it turned out to be not good. Can anyone recommend me some good and authentic source of...

02 March 2015 1,962 1 View

Will an inter-species fusion antibody be as effective as a wild type?

If we fuse conserved region of antibody from mouse to a variable region from rat will the fusion antibody work with similar efficiency? Or is there any other method to murinise the sequence of...

08 September 2014 6,348 6 View

What is the maximum molecular weight shift after phosphorylation?

During my Masters my teacher told me there is around 20 kDa molecular weight shift after excessive phosphorylation of a protein.. Problem is I forgot the name of the protein. Does anybody remember...

07 August 2014 7,418 2 View

No title

02 March 2021 324 2 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How to select from various invitations from Scientific Boards in conferences and proceedings?

How to select from various invitations from Scientific Boards in conferences and proceedings? How to spot the ones with high impact and genuine? Can we spot valuable events?

02 February 2021 8,187 1 View

What is the most appropriate design (statistic test) to assess the toxicity of 2 different compounds at 6 different concentrations and 2 conditions?

10 January 2021 8,077 3 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

Is it easy to collect a conference certificate?

share your experience please.

01 January 2021 5,084 4 View

Is training really prepare you for a conference?

Does training really prepare you for a conference?

01 January 2021 1,601 2 View

What is the procedure of attending international conference and seminar?

What is the procedure of attending international conference and seminar?

01 January 2021 7,733 1 View