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What is the proper dosage for supplemental biotin in primary cell culture?

I am trying to overexpress a TurboID construct in mouse primary culture cells for validation experiments. Unfortunately the transfection of this construct appears to be killing my cells while...

26 February 2024 8,929 2 View

How to measure the DMA peak of Propolyene?

Hello every expert, When I did the DMA expertment for propolyene, I can't not get the normal spectrum. I intended to find the tg peak of PP, but it was not easy to have the beautiful peak in this...

22 February 2024 7,216 0 View

Repeated measures in three groups: which test to use?

Hello, I have an experiment in which three groups of 15 participants (disorder 1, disorder 2, control group) underwent an exercise test. I have 14 measurement points (each with a 5-min interval)....

13 December 2023 1,508 4 View

After qPCR analysis of gene expression, when is difference in relative fold change actually relevant?

During work for my undergrad thesis, I've examined and compared gene expression of stress-induced genes in plants challenged with a fungal infection. I have calculated the relative fold change and...

22 November 2023 5,517 3 View

Mushroom cultivation: can we reduce water needed for entire process while also providing drinking water for billions of people?

Our social enterprise's focus [0] is mushroom cultivation in developing countries where easy access to water cannot be assumed: 20%, globally, still have to walk away from their premise to get it...

31 October 2023 9,855 0 View

What is the best way to fix cells for non-permeabilized stains?

Hello, I need to stain some primary culture cells in permeabilized and non-permeabilized conditions to test if a protein is localizing to the exterior of the cell membrane. Our existing fixation...

20 September 2023 5,142 3 View

Why did I get a collection of 5 genes when I search for the gene data of SMN1?

This is what I got when I searched for SMN1 (survival of motor neuron 1) gene: https://www.ncbi.nlm.nih.gov/gene/6606 However, when I downloaded the sequence data, I found there are 5 gene...

14 August 2023 8,121 0 View

Where can I find PET microspheres?

I am looking for PET microspheres for a research project that I am conducting for my dissertation.

25 September 2022 1,788 2 View

Physical Explanation of Phased Array Antenna Directivity on Receive?

Phased array antenna directivity is generally explained on transmit. For example, a uniform linear array (ULA) with element spacing of half a wavelength (scanned to boresight) has a directivity...

26 July 2022 6,703 10 View

Has anyone performed ELISA for Host Cell Proteins on recombinant IL-12 expressed from Pichia Pastoris with successful results?

Has anyone performed ELISA for Host Cell Proteins on recombinant IL-12 expressed from Pichia Pastoris with successful results? Also, has anyone performed mass spec or light scattering on the same...

07 June 2022 6,042 0 View

How to develop investments in renewable energy sources?

08 August 2024 5,112 3 View

Do you think can be any diamond in A type eclogites?

I want to know more about diamond ore deposits in world.

08 August 2024 1,514 0 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,705 0 View

Enhancing Critical Thinking Skills for Slow Learners: A Review of Empirical Studies?

to identify themes in question with APA style references

07 August 2024 2,239 5 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Do you think can be any gas and oil bearing rocks in Eastern part of Iran?

I want to know more about petroleum deposits in Iran.

02 August 2024 8,725 3 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View