Has anyone performed ELISA for Host Cell Proteins on recombinant IL-12 expressed from Pichia Pastoris with successful results?
Also, has anyone performed mass spec or light scattering on the same protein to determine the molecular weight?
I want to know more about Uranium ore deposits in world.
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I want to know more about diamond ore deposits in world.
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We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
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If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
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I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
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What are a “Farmers Producer Organization” (FPO) and its essential features?
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I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
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How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
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I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
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How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
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I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
through genetic modification or metabolic engineering? Is it helpful, good for health ?
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Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
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I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...
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is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?
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I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...
26 June 2024 3,735 3 View
Hello everyone, I am isolating PBMCs and stimulating them using different stimulation conditions. my question is, I want to add IL-2 to the medium for better viability after stimulation, Would you...
16 June 2024 928 1 View
I have been using Zeocin marker for target protein expression to verify integration in Pichia system. However, I would like to remove Zeocin marker since it's antibiotic related and not ideal for...
15 June 2024 3,851 2 View
The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10...
13 June 2024 2,590 4 View
I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method other than cryopreservation of spore for a short term...
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