Is multivariate regression analysis appropriate in analysing ordinal, observational data within a non-inferiority study?

Good evening, In reply to the peer review of a paper up for publication, I've been asked to consider multivariate regression analysis. In analysing patient data we looked to show the safety of...

20 December 2020 8,426 5 View

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Engineering geologists may know that the originators of the empirical (a posteriori) Q-system method of rock mass classification for single-shell tunnel and cavern support estimation, were Barton...

13 December 2020 5,103 3 View

Does anyone have good examples of how aesthetic standards have influenced creative design in building structures?

My research is looking at the influence of creative design and technical standards on the development of architecture during a period of history. This has relevance to the modern day in terms of a...

05 November 2020 5,214 6 View

Using both dichotomous and polytomous indicators for a latent variable in a path model?

Hi all! I'm conducting a study where I have a designed a path model wherein most latent variables have polytomous indicators (ordinal data). However, one instrument I intend on using measures a...

26 March 2020 5,967 3 View

Effect of excess RBC in PRP injections?

Ive been using sodium citrate and acd-a collection tubes to spin blood and extract platelets for PRP. The collection tubes I use do not have the separating gel. Ive noticed that in order to get...

04 February 2020 3,177 3 View

Is the calculation of AVE and composite reliability after EFA recommended?

Greetings! The link to the Todd Grande tutorial below demonstrating the calculation of AVE and composite reliability to factor loadings appears to be popular viewing. My question is would you...

08 December 2019 711 4 View

Can you offer arguments for and against using exploratory factor analysis deductively?

Greetings! Please offer your thoughts on my general question regarding factor analysis. If one is purely concerned with demonstrating convergent and discriminant construct validity is the use of...

29 August 2019 4,589 3 View

How to identify the residues that have interaction from autodock or other docking website results?

28 August 2019 655 2 View

Beginners advice please - testing relationships between latent constructs for the first time?

Greetings. Following a qualitative exploration of my research topic, I am embarking on the quantitative follow-up phase of my research. I want to test the relationships between 10 predictor...

04 March 2019 4,279 3 View

Any suggestions for software that will interpolate test hole data in 3D?

I'm looking at Earth Volumetric Studio, Arc Hydro Groundwater with Subsurface Analyst add on, and Hydro GeoAnalyst. I'm working with large testhole data sets and want to create 2D cross sections...

24 February 2019 7,157 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View