Biofumigants and antagonists are applied for management of stem rot disease caused by Rhizoctonia solani Kuhn in carnation. The antagonist activity of the native biocontol agents particularly Trichoderma species, T. viride, T. harzianum, T. virens, T. koeinigii and three bacterial strains Bacillus subtilis, Pseudomonas fluorescens and Bacillus spp. control infection of Rhizoctonia solani.Six mm dia. bits of each antagonistic fungi can be taken from vigorously
growing margins and are transferred aseptically to one side of solidified PDA medium in the Petri plates.Hot water extracts of different plants Melia azedarach, (Darek- seed extract) and leaf extracts of Eucalyptus globules (Safeda), Roylea elegance (Karu), Vitex negundo (Banna), Adhatoda vasica (Basuti), Tagetes erecta (Marigold), Murraya koenigii (Currypatta), Mentha arvensis (Pudina),Cannabis sativa (Bhang) can be used for bio-fumigation. Biofumigation is also done by commercial formulations for neem at 3 and 5 % in vitro against Rhizoctonia solani. Biofumigants (cruciferous) effects crop residues on disease and plant growth parameters and survival of pathogen and soil microflora, neem leaves and garlic leaves are reported to be very effective in controlling
Fusarium, Rhizopus and Curvularia spp. causing seed rot of mustard Use of
plant extract as an antifungal substance against Rhizoctonia solani is done by using Trichoderma harzianum, T. viride along with neemgold or neemazal and cruciferous residues particularly of cauliflower and cabbage (2g/kg of soil) can be a good alternative source to chemicals in managing stem rot of carnation in
integrated form with minimum impact on the soil and environment.
Biofumigation by augmenting deoiled mustard oil cake do inhibit pathogenic fungi but also affect Trichoderma and other PGPR. The best part it that being fast growing Trichoderma colonizes soil faster in absence of completion and fortifies itself against pathogenic fungi. Lot of field work has been reported from Pant Nagar University.