How to Remove different version of atom from pdb Crystal Structure for Docking Preparation?

I am new to Docking and MD, and currently try to catch on it. I try one of the webserver for docking, but unfortunately not working. it says " Your PDB contains multiple forms of the same residue...

08 February 2021 7,426 3 View

What makes a strong research question?

A good research question is essential to guide your research paper, project, or thesis. It pinpoints exactly what you want to find out and gives your work a clear focus and purpose.

25 December 2020 6,145 4 View

Does Neighbor-Joining (NJ) clustering method suitable to cluster the water quality data?

Dear researchers, I'm using a software called PAST to analyze my multivariate water quality data. For clustering the sampling stations using 14 water quality variables, I found that NJ with...

27 July 2020 2,972 3 View

Do you know what is TCGA database?

Hello, Do any one know how TCGA database works? any tutorial to show ho to use it and interpret the data? Also, any idea about any database website that shows the expression level of specific...

29 August 2016 1,493 3 View

Why I couldn't detect it ?

18 June 2016 2,081 3 View

What is the relation between the number of people and the temperature in the room?

05 June 2016 8,300 8 View

Hello,I am looking for a database bioinformatic tool to show the expression of microRNA in normal and malignant breast tissue?

Hello,I am looking for a database bioinformatic tool to show the expression of microRNA in normal and malignant breast tissue, Thanks

11 May 2016 4,951 3 View

Is there any difference on primary antibody binding region?

27 January 2016 6,179 2 View

How can I distinguish between Ce4+(CeO2) & Ce3+(Ce2O3) as promoter in the catalysts?

09 June 2015 1,320 6 View

Why can't Dragendorff's reagent precipitate caffeine?

See above

26 May 2014 232 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How to estimation of reverse-transcription on tissue?

I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...

08 February 2021 1,957 4 View