Hello all,
I'm trying to isolate some cells from pig gingival tissue, I'm working under hood and every thing is sterile and clean, I wash the tissue 5 times with pbs containing 10x pen/strep before starting but every time I'm getting some contamination next day, the media becomes cloudy with some white spots and there are many small black dots when looking under microscope. how can I solve this problem?
@ Dear Sevda Pouraghaei we used pen/strep as an antibiotic against bacteria and you can use Amphotericin B for prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast. or you can culture this bacteria or fungi in agar plate and after antibiogram can find correct amount of antibiotic.
I think this bacteria due to you find it next day. So You can used ampicillin as an antibiotic against bacteria and you can use Amphotericin B for prevents fungal contamination of cell cultures.
Article Intestinal Stem Cell Isolation and Culture in a Porcine Mode...
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