Sevda Pouraghaei

6 Questions 13 Answers 0 Followers

Questions related from Sevda Pouraghaei

Any ideas on the antibiotics used for isolating cells from pig oral tissue?

Hello all, I'm trying to isolate some cells from pig gingival tissue, I'm working under hood and every thing is sterile and clean, I wash the tissue 5 times with pbs containing 10x pen/strep...

12 October 2018 5,858 3 View

How to avoid contamination while isolating cells from pig oral tissue?

Hello all, I'm trying to isolate some cells from pig gingival tissue, I'm working under hood and every thing is sterile and clean, I wash the tissue 5 times with pbs containing 10x pen/strep...

04 October 2018 829 3 View

What happens when matrigel becomes gel?

I am trying to culture my stem cells in a 3D condition in the presence of Matrigel. Some times I am not fast enough and can't handle Matrigel properly; therefore, it becomes gel and doesn't mix...

13 November 2017 5,592 3 View

Looking for resean of low embryonic stem cell viability in hydrogel?

I am trying to culture mouse embryonic stem cells in chitosan hydrogel. but the viability is very low. what can be the reason and how can I increase it? I am trying to culture them in the form of...

10 April 2017 6,714 10 View

How can I detach the encapsulated embryonic stem cells from the chitosan hydrogel?

I am trying to culture mouse embryonic stem cells in 3D chitosan hydrogel but can't harvest cells from hydrogel in order to do some assays like cell count or cell viability,etc. is there any...

04 March 2017 5,267 9 View

DMEM (low glucose) , DMEM (high glucose),DMEM/f12 for embryonic stem cell culture?

Dear friends, which of the following medium is better for culturing mouse embryonic stem cells? DMEM (low glucose) , DMEM (high glucose),DMEM/f12

29 November 2016 2,430 6 View